A high density genetic map of tobacco (Nicotiana tabacum L.) obtained from large scale microsatellite marker development.

ABSTRACT: Tobacco (Nicotiana tabacum L.) is a species in the large family of the Solanaceae and is important as an agronomic crop and as a model system in plant biotechnology. Despite its importance, only limited molecular marker resources are available that can be used for genome analysis, genetic mapping and breeding. We report here on the development and characterization of 5,119 new and functional microsatellite markers and on the generation of a high-resolution genetic map for the tetraploid tobacco genome. The genetic map was generated using an F2 mapping population derived from the intervarietal cross of Hicks Broadleaf × Red Russian and merges the polymorphic markers from this new set with those from a smaller set previously used to produce a lower density map. The genetic map described here

SUBMITTER: Bindler G 

PROVIDER: S-EPMC3114088 | biostudies-literature | 2011 Jul

REPOSITORIES: biostudies-literature