Multivalent display of proteins on viral nanoparticles using molecular recognition and chemical ligation strategies.

ABSTRACT: Multivalent display of heterologous proteins on viral nanoparticles forms a basis for numerous applications in nanotechnology, including vaccine development, targeted therapeutic delivery, and tissue-specific bioimaging. In many instances, precise placement of proteins is required for optimal functioning of the supramolecular assemblies, but orientation- and site-specific coupling of proteins to viral scaffolds remains a significant technical challenge. We have developed two strategies that allow for controlled attachment of a variety of proteins on viral particles using covalent and noncovalent principles. In one strategy, an interaction between domain 4 of anthrax protective antigen and its receptor was used to display multiple copies of a target protein on virus-like particles. In the o

SUBMITTER: Venter PA 

PROVIDER: S-EPMC3114102 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature