Cross-talk between interferon-γ and hedgehog signaling regulates adipogenesis.

ABSTRACT:

Objective

T cells and level of the cytokine interferon-γ (IFN-γ) are increased in adipose tissue in obesity. Hedgehog (Hh) signaling has been shown to potently inhibit white adipocyte differentiation. In light of recent findings in neurons that IFN-γ and Hh signaling cross-talk, we examined their potential interaction in the context of adipogenesis.

Research design and methods

We used Hh reporter cells, cell lines, and primary adipocyte differentiation models to explore costimulation of IFN-γ and Hh signaling. Genetic dissection using Ifngr1(-/-) and Stat1(-/-) mouse embryonic fibroblasts, and ultimately, anti-IFN-γ neutralization and expression profiling in obese mice and humans, respectively, were used to place the findings into the in vivo context.

Results

T-cell s

SUBMITTER: Todoric J 

PROVIDER: S-EPMC3114396 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature