Identification of factors required for meristem function in Arabidopsis using a novel next generation sequencing fast forward genetics approach.

ABSTRACT:

Background

Phenotype-driven forward genetic experiments are powerful approaches for linking phenotypes to genomic elements but they still involve a laborious positional cloning process. Although sequencing of complete genomes now becomes available, discriminating causal mutations from the enormous amounts of background variation remains a major challenge.

Method

To improve this, we developed a universal two-step approach, named 'fast forward genetics', which combines traditional bulk segregant techniques with targeted genomic enrichment and next-generation sequencing technology

Results

As a proof of principle we successfully applied this approach to two Arabidopsis mutants and identified a novel factor required for stem cell activity.

Conclusion

We demonstrated

SUBMITTER: Mokry M 

PROVIDER: S-EPMC3114748 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature