Premitotic assembly of human CENPs -T and -W switches centromeric chromatin to a mitotic state.

ABSTRACT: Centromeres are differentiated chromatin domains, present once per chromosome, that direct segregation of the genome in mitosis and meiosis by specifying assembly of the kinetochore. They are distinct genetic loci in that their identity in most organisms is determined not by the DNA sequences they are associated with, but through specific chromatin composition and context. The core nucleosomal protein CENP-A/cenH3 plays a primary role in centromere determination in all species and directs assembly of a large complex of associated proteins in vertebrates. While CENP-A itself is stably transmitted from one generation to the next, the nature of the template for centromere replication and its relationship to kinetochore function are as yet poorly understood. Here, we investigate the assembly a

SUBMITTER: Prendergast L 

PROVIDER: S-EPMC3114758 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature