Sequence analysis of fusion protein gene of Newcastle disease virus isolated from outbreaks in Egypt during 2006.

ABSTRACT:

Background

Newcastle disease virus represents APMV-1 and is the most characterized among all APMV types. The F protein cleavage site sequence is a well-characterized determinant of NDV pathogenicity in chickens. In this study, the sequences of fusion protein (F) gene of three Newcastle disease virus (NDV) strains isolated from outbreak in chickens in the Al-Sharkia province of Egypt in 2006 were determined.

Findings

The viral genomic RNAs were extracted from the infective allantoic fluid and F gene is amplified using primer sets designed from the available sequences of NDV strains from GenBank. The pathogenicity of NDV strains was determined by three internationally recognized tests mean death time, intracerebral pathogenicity index, and intravenous pathogenicity index. The

SUBMITTER: Mohamed MH 

PROVIDER: S-EPMC3114776 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature