Highly efficient chemoenzymatic synthesis of β1-3-linked galactosides.

ABSTRACT: A novel D-galactosyl-β1-3-N-acetyl-D-hexosamine phosphorylase cloned from Bifidobacterium infantis (BiGalHexNAcP) was used with a recombinant E. coli K-12 galactokinase (GalK) for efficient one-pot two-enzyme synthesis of T-antigens, galacto-N-biose (Galβ1-3GalNAc), lacto-N-biose (Galβ1-3GlcNAc), and their derivatives.

SUBMITTER: Yu H 

PROVIDER: S-EPMC3114946 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature