An effective assay for high cellular resolution time-lapse imaging of sensory placode formation and morphogenesis.

ABSTRACT:

Background

The vertebrate peripheral nervous system contains sensory neurons that arise from ectodermal placodes. Placodal cells ingress to move inside the head to form sensory neurons of the cranial ganglia. To date, however, the process of placodal cell ingression and underlying cellular behavior are poorly understood as studies have relied upon static analyses on fixed tissues. Visualizing placodal cell behavior requires an ability to distinguish the surface ectoderm from the underlying mesenchyme. This necessitates high resolution imaging along the z-plane which is difficult to accomplish in whole embryos. To address this issue, we have developed an imaging system using cranial slices that allows direct visualization of placode formation.

Results

We demonstrate an effect

SUBMITTER: Shiau CE 

PROVIDER: S-EPMC3115907 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature