MMP28 (epilysin) as a novel promoter of invasion and metastasis in gastric cancer.

ABSTRACT:

Background

The purpose of this study was to investigate invasion and metastasis related genes in gastric cancer.

Methods

The transwell migration assay was used to select a highly invasive sub-line from minimally invasive parent gastric cancer cells, and gene expression was compared using a microarray. MMP28 upregulation was confirmed using qRT-PCR. MMP28 immunohistochemistry was performed in normal and gastric cancer specimens. Invasiveness and tumor formation of stable cells overexpressing MMP28 were tested in vitro and in vivo.

Results

MMP28 was overexpressed in the highly invasive sub-cell line. Immunohistochemistry revealed MMP28 expression was markedly increased in gastric carcinoma relative to normal epithelia, and was significantly associated with depth of tumo

SUBMITTER: Jian P 

PROVIDER: S-EPMC3115915 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature