Agrobacterium-mediated transformation of safflower and the efficient recovery of transgenic plants via grafting.

ABSTRACT:

Background

Safflower (Carthamus tinctorius L.) is a difficult crop to genetically transform being susceptible to hyperhydration and poor in vitro root formation. In addition to traditional uses safflower has recently emerged as a broadacre platform for the production of transgenic products including modified oils and pharmaceutically active proteins. Despite commercial activities based on the genetic modification of safflower, there is no method available in the public domain describing the transformation of safflower that generates transformed T1 progeny.

Results

An efficient and reproducible protocol has been developed with a transformation efficiency of 4.8% and 3.1% for S-317 (high oleic acid content) and WT (high linoleic acid content) genotypes respectively. An improve

SUBMITTER: Belide S 

PROVIDER: S-EPMC3115923 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature