A DNMT3B alternatively spliced exon and encoded peptide are novel biomarkers of human pluripotent stem cells.

ABSTRACT: A major obstacle in human stem cell research is the limited number of reagents capable of distinguishing pluripotent stem cells from partially differentiated or incompletely reprogrammed derivatives. Although human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) express numerous alternatively spliced transcripts, little attention has been directed at developing splice variant-encoded protein isoforms as reagents for stem cell research. In this study, several genes encoding proteins involved in important signaling pathways were screened to detect alternatively spliced transcripts that exhibited differential expression in pluripotent stem cells (PSCs) relative to spontaneously differentiated cells (SDCs). Transcripts containing the alternatively spliced exon 10 of the

SUBMITTER: Gopalakrishna-Pillai S 

PROVIDER: S-EPMC3115941 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature