Molecular cloning, characterization and expression analysis of two members of the Pht1 family of phosphate transporters in Glycine max.

ABSTRACT:

Background

Phosphorus is one of the macronutrients essential for plant growth and development. The acquisition and translocation of phosphate are pivotal processes of plant growth. In a large number of plants, phosphate uptake by roots and translocation within the plant are presumed to occur via a phosphate/proton cotransport mechanism.

Principal findings

We cloned two cDNAs from soybean (Glycine max), GmPT1 and GmPT2, which show homology to the phosphate/proton cotransporter PHO84 from the budding yeast Saccharomyces cerevisiae. The amino acid sequence of the products predicted from GmPT1 and GmPT2 share 61% and 63% identity, respectively, with the PHO84 in amino acid sequence. The deduced structure of the encoded proteins revealed 12 membrane-spanning domains with a centra

SUBMITTER: Wu Z 

PROVIDER: S-EPMC3115949 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature