Transcriptome profiling of whole blood cells identifies PLEK2 and C1QB in human melanoma.

ABSTRACT:

Background

Developing analytical methodologies to identify biomarkers in easily accessible body fluids is highly valuable for the early diagnosis and management of cancer patients. Peripheral whole blood is a "nucleic acid-rich" and "inflammatory cell-rich" information reservoir and represents systemic processes altered by the presence of cancer cells.

Methodology/principal findings

We conducted transcriptome profiling of whole blood cells from melanoma patients. To overcome challenges associated with blood-based transcriptome analysis, we used a PAXgene™ tube and NuGEN Ovation™ globin reduction system. The combined use of these systems in microarray resulted in the identification of 78 unique genes differentially expressed in the blood of melanoma patients. Of these, 68 gen

SUBMITTER: Luo Y 

PROVIDER: S-EPMC3115966 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature