Nkx6.2 synergizes with Cdx-2 in stimulating proglucagon gene expression.

ABSTRACT:

Aim

To investigate whether the transactivator of the proglucagon gene (Gcg), Cdx-2, synergizes with other transcription factors in stimulating Gcg expression and the trans-differentiation of Gcg-expressing cells.

Methods

We conducted affinity chromatography to identify proteins that interact with Cdx-2, using GST-tagged Cdx-2 against cell lysates from pancreatic InR1-G9 and intestinal GLUTag cell lines. This was followed by a mass-spectrometry analysis. From a potential Cdx-2 interaction protein identified, we examined its expression in pancreatic and gut endocrine cells, confirmed its interaction with Cdx-2 by GST-pull down and determined its effect in provoking Gcg expression in cell lines that do not express endogenous Gcg.

Results

We identified 18 potential Cdx-2

SUBMITTER: Wang PX 

PROVIDER: S-EPMC3116010 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature