Building a model: developing genomic resources for common milkweed (Asclepias syriaca) with low coverage genome sequencing.

ABSTRACT:

Background

Milkweeds (Asclepias L.) have been extensively investigated in diverse areas of evolutionary biology and ecology; however, there are few genetic resources available to facilitate and compliment these studies. This study explored how low coverage genome sequencing of the common milkweed (Asclepias syriaca L.) could be useful in characterizing the genome of a plant without prior genomic information and for development of genomic resources as a step toward further developing A. syriaca as a model in ecology and evolution.

Results

A 0.5× genome of A. syriaca was produced using Illumina sequencing. A virtually complete chloroplast genome of 158,598 bp was assembled, revealing few repeats and loss of three genes: accD, clpP, and ycf1. A nearly complete rDNA cistron (18S

SUBMITTER: Straub SC 

PROVIDER: S-EPMC3116503 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature