Amino acid substitutions at the major insertion loop of Candida albicans sterol 14alpha-demethylase are involved in fluconazole resistance.

ABSTRACT:

Background

In the fungal pathogen Candida albicans, amino acid substitutions of 14alpha-demethylase (CaErg11p, CaCYP51) are associated with azole antifungals resistance. This is an area of research which is very dynamic, since the stakes concern the screening of new antifungals which circumvent resistance. The impact of amino acid substitutions on azole interaction has been postulated by homology modeling in comparison to the crystal structure of Mycobacterium tuberculosis (MT-CYP51). Modeling of amino acid residues situated between positions 428 to 459 remains difficult to explain to date, because they are in a major insertion loop specifically present in fungal species.

Methodology/principal finding

Fluconazole resistance of clinical isolates displaying Y447H and V456I nov

SUBMITTER: Alvarez-Rueda N 

PROVIDER: S-EPMC3116904 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature