Transcriptomic and proteomic profiling of maize embryos exposed to camptothecin.

ABSTRACT:

Background

Camptothecin is a plant alkaloid that specifically binds topoisomerase I, inhibiting its activity and inducing double stranded breaks in DNA, activating the cell responses to DNA damage and, in response to severe treatments, triggering cell death.

Results

Comparative transcriptomic and proteomic analyses of maize embryos that had been exposed to camptothecin were conducted. Under the conditions used in this study, camptothecin did not induce extensive degradation in the genomic DNA but induced the transcription of genes involved in DNA repair and repressed genes involved in cell division. Camptothecin also affected the accumulation of several proteins involved in the stress response and induced the activity of certain calcium-dependent nucleases. We also detected

SUBMITTER: Sanchez-Pons N 

PROVIDER: S-EPMC3118180 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature