Identification of new, emerging HIV-1 unique recombinant forms and drug resistant viruses circulating in Cameroon.

ABSTRACT:

Background

The HIV epidemic in Cameroon is characterized by a high degree of viral genetic diversity with circulating recombinant forms (CRFs) being predominant. The goal of our study was to determine recent trends in virus evolution and emergence of drug resistance in blood donors and HIV positive patients.

Methodology

Blood specimens of 73 individuals were collected from three cities and a few villages in Cameroon and viruses were isolated by co-cultivation with PBMCs. Nested PCR was performed for gag p17 (670 bp) pol (840 bp) and Env gp41 (461 bp) genes. Sequences were phylogenetically analyzed using a reference set of sequences from the Los Alamos database.

Results

Phylogenetic analysis based on partial sequences revealed that 65% (n = 48) of strains were CRF02_AG

SUBMITTER: Ragupathy V 

PROVIDER: S-EPMC3118203 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature