Molecular and process design for rotavirus-like particle production in Saccharomyces cerevisiae.

ABSTRACT:

Background

Virus-like particles (VLP) have an increasing range of applications including vaccination, drug delivery, diagnostics, gene therapy and nanotechnology. These developments require large quantities of particles that need to be obtained in efficient and economic processes. Production of VLP in yeast is attractive, as it is a low-cost protein producer able to assemble viral structural proteins into VLP. However, to date only single-layered VLP with simple architecture have been produced in this system. In this work, the first steps required for the production of rotavirus-like particles (RLP) in S. cerevisiae were implemented and improved, in order to obtain the recombinant protein concentrations required for VLP assembly.

Results

The genes of the rotavirus structural

SUBMITTER: Rodriguez-Limas WA 

PROVIDER: S-EPMC3118324 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature