Increasing gene dosage greatly enhances recombinant expression of aquaporins in Pichia pastoris.

ABSTRACT:

Background

When performing functional and structural studies, large quantities of pure protein are desired. Most membrane proteins are however not abundantly expressed in their native tissues, which in general rules out purification from natural sources. Heterologous expression, especially of eukaryotic membrane proteins, has also proven to be challenging. The development of expression systems in insect cells and yeasts has resulted in an increase in successful overexpression of eukaryotic proteins. High yields of membrane protein from such hosts are however not guaranteed and several, to a large extent unexplored, factors may influence recombinant expression levels. In this report we have used four isoforms of aquaporins to systematically investigate parameters that may affect pro

SUBMITTER: Norden K 

PROVIDER: S-EPMC3118338 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature