Latent Membrane Protein 1 as a molecular adjuvant for single-cycle lentiviral vaccines.

ABSTRACT:

Background

Molecular adjuvants are a promising method to enhance virus-specific immune responses and protect against HIV-1 infection. Immune activation by ligands for receptors such as CD40 can induce dendritic cell activation and maturation. Here we explore the incorporation of two CD40 mimics, Epstein Barr Virus gene LMP1 or an LMP1-CD40 chimera, into a strain of SIV that was engineered to be limited to a single cycle of infection.

Results

Full length LMP1 or the chimeric protein LMP1-CD40 was cloned into the nef-locus of single-cycle SIV. Human and Macaque monocyte derived macrophages and DC were infected with these viruses. Infected cells were analyzed for activation surface markers by flow cytometry. Cells were also analyzed for secretion of pro-inflammatory cytokines I

SUBMITTER: Gupta S 

PROVIDER: S-EPMC3118346 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature