New cell separation technique for the isolation and analysis of cells from biological mixtures in forensic caseworks.

ABSTRACT:

Aim

To isolate mucosal cells of the perpetrator in a sexual assault case from a complex mixture of his mucosal cells and the victim's skin by micromanipulation prior to genomic analysis.

Methods

To capture and analyze mucosal cells we used the micromanipulation with on-chip low volume polymerase chain reaction (LV-PCR). Consensus DNA profiles were generated from 5 replicate experiments.

Results and conclusions

We validated the use of micromanipulation with on-chip LV-PCR for genomic analysis of complex biological mixtures in a fatal rape case. The perpetrator's mucosal cells were captured from nipple swabs of the victim, and a single-source DNA profile was generated from cell mixtures. These data suggest that micromanipulation with on-chip LV-PCR is an effective foren

SUBMITTER: Li CX 

PROVIDER: S-EPMC3118714 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature