Proteomics in diagnostic pathology: profiling and imaging proteins directly in tissue sections.

ABSTRACT: Direct tissue profiling and imaging mass spectrometry (MS) provide a molecular assessment of numerous expressed proteins within a tissue sample. MALDI MS (matrix-assisted laser desorption ionization) analysis of thin tissue sections results in the visualization of 500 to 1000 individual protein signals in the molecular weight range from 2000 to over 200,000. These signals directly correlate with protein distribution within a specific region of the tissue sample. The systematic investigation of the section allows the construction of ion density maps, or specific molecular images, for virtually every signal detected in the analysis. Ultimately, hundreds of images, each at a specific molecular weight, may be obtained. To date, profiling and imaging MS has been applied to multiple diseased tis

SUBMITTER: Chaurand P 

PROVIDER: S-EPMC3118833 | biostudies-literature | 2004 Oct

REPOSITORIES: biostudies-literature