Small interference RNA targeting tissue factor inhibits human lung adenocarcinoma growth in vitro and in vivo.

ABSTRACT:

Background

The human coagulation trigger tissue factor (TF) is overexpressed in several types of cancer and involved in tumor growth, vascularization, and metastasis. To explore the role of TF in biological processes of lung adenocarcinoma, we used RNA interference (RNAi) technology to silence TF in a lung adenocarcinoma cell line A549 with high-level expression of TF and evaluate its antitumor effects in vitro and in vivo.

Methods

The specific small interfering RNA (siRNA) designed for targeting human TF was transfected into A549 cells. The expression of TF was detected by reverse transcription-PCR and Western blot. Cell proliferation was measured by MTT and clonogenic assays. Cell apoptosis was assessed by flow cytometry. The metastatic potential of A549 cells was determin

SUBMITTER: Xu C 

PROVIDER: S-EPMC3118969 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature