Genome-wide expression profiling of the response to short-term exposure to fluconazole in Cryptococcus neoformans serotype A.

ABSTRACT:

Background

Fluconazole (FLC), a triazole antifungal drug, is widely used for the maintenance therapy of cryptococcal meningoencephalitis, the most common opportunistic infection in AIDS patients. In this study, we examined changes in the gene expression profile of the C. neoformans reference strain H99 (serotype A) following FLC treatment in order to investigate the adaptive cellular responses to drug stress.

Results

Simultaneous analysis of over 6823 transcripts revealed that 476 genes were responsive to FLC. As expected up-regulation of genes involved in ergosterol biosynthesis was observed, including the azole target gene ERG11 and ERG13, ERG1, ERG7, ERG25, ERG2, ERG3 and ERG5. In addition, SRE1 which is a gene encoding a well-known regulator of sterol homeostasis in C. n

SUBMITTER: Florio AR 

PROVIDER: S-EPMC3119188 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature