Selective enrichment and identification of azide-tagged cross-linked peptides using chemical ligation and mass spectrometry.

ABSTRACT: Protein-protein interaction is one of the key regulatory mechanisms for controlling protein function in various cellular processes. Chemical cross-linking coupled with mass spectrometry has proven to be a powerful method not only for mapping protein-protein interactions of all natures, including weak and transient ones, but also for determining their interaction interfaces. One critical challenge remaining in this approach is how to effectively isolate and identify cross-linked products from a complex peptide mixture. In this work, we have developed a novel strategy using conjugation chemistry for selective enrichment of cross-linked products. An azide-tagged cross-linker along with two biotinylated conjugation reagents were designed and synthesized. Cross-linking of model peptides and cyt

SUBMITTER: Vellucci D 

PROVIDER: S-EPMC3119349 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature