Alternative exon 9-encoded relay domains affect more than one communication pathway in the Drosophila myosin head.

ABSTRACT: We investigated the biochemical and biophysical properties of one of the four alternative regions within the Drosophila myosin catalytic domain: the relay domain encoded by exon 9. This domain of the myosin head transmits conformational changes in the nucleotide-binding pocket to the converter domain, which is crucial to coupling catalytic activity with mechanical movement of the lever arm. To study the function of this region, we used chimeric myosins (IFI-9b and EMB-9a), which were generated by exchange of the exon 9-encoded domains between the native embryonic body wall (EMB) and indirect flight muscle isoforms (IFI). Kinetic measurements show that exchange of the exon 9-encoded region alters the kinetic properties of the myosin S1 head. This is reflected in reduced values for ATP-induc

SUBMITTER: Bloemink MJ 

PROVIDER: S-EPMC3119900 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature