A large, consistent plasma proteomics data set from prospectively collected breast cancer patient and healthy volunteer samples.

ABSTRACT:

Background

Variability of plasma sample collection and of proteomics technology platforms has been detrimental to generation of large proteomic profile datasets from human biospecimens.

Methods

We carried out a clinical trial-like protocol to standardize collection of plasma from 204 healthy and 216 breast cancer patient volunteers. The breast cancer patients provided follow up samples at 3 month intervals. We generated proteomics profiles from these samples with a stable and reproducible platform for differential proteomics that employs a highly consistent nanofabricated ChipCube™ chromatography system for peptide detection and quantification with fast, single dimension mass spectrometry (LC-MS). Protein identification is achieved with subsequent LC-MS/MS analysis employing

SUBMITTER: Riley CP 

PROVIDER: S-EPMC3120690 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature