Live in vivo imaging of Egr-1 promoter activity during neonatal development, liver regeneration and wound healing.

ABSTRACT:

Background

The zinc finger transcription factor Egr-1 (Early growth response 1) is central to several growth factors and represents an important activator of target genes not only involved in physiological processes like embryogenesis and neonatal development, but also in a variety of pathophysiological processes, for example atherosclerosis or cancer. Current options to investigate its transcription and activation in vivo are end-point measurements that do not provide insights into dynamic changes in the living organism.

Results

We developed a transgenic mouse (Egr-1-luc) in which the luciferase reporter gene is under the control of the murine Egr-1 promoter providing a versatile tool to study the time course of Egr-1 activation in vivo. In neonatal mice, bioluminescence im

SUBMITTER: Dussmann P 

PROVIDER: S-EPMC3120781 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature