The first insight into the tissue specific taxus transcriptome via Illumina second generation sequencing.

ABSTRACT:

Background

Illumina second generation sequencing is now an efficient route for generating enormous sequence collections that represent expressed genes and quantitate expression level. Taxus is a world-wide endangered gymnosperm genus and forms an important anti-cancer medicinal resource, but the large and complex genomes of Taxus have hindered the development of genomic resources. The research of its tissue-specific transcriptome is absent. There is also no study concerning the association between the plant transcriptome and metabolome with respect to the plant tissue type.

Methodology/principal findings

We performed the de novo assembly of Taxus mairei transcriptome using Illumina paired-end sequencing technology. In a single run, we produced 13,737,528 sequencing reads cor

SUBMITTER: Hao da C 

PROVIDER: S-EPMC3120849 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature