Inhibition of plasminogen activator inhibitor-1 restores skeletal muscle regeneration in untreated type 1 diabetic mice.

ABSTRACT:

Objective

Type 1 diabetes leads to impairments in growth, function, and regenerative capacity of skeletal muscle; however, the underlying mechanisms have not been clearly defined.

Research design and methods

With the use of Ins2(WT/C96Y) mice (model of adolescent-onset type 1 diabetes), muscle regeneration was characterized in terms of muscle mass, myofiber size (cross-sectional area), and protein expression. Blood plasma was analyzed for glucose, nonesterified fatty acids, insulin, and plasminogen activator inhibitor-1 (PAI-1). PAI-039, an effective inhibitor of PAI-1, was orally administered to determine if PAI-1 was attenuating muscle regeneration in Ins2(WT/C96Y) mice.

Results

Ins2(WT/C96Y) mice exposed to 1 or 8 weeks of untreated type 1 diabetes before chemicall

SUBMITTER: Krause MP 

PROVIDER: S-EPMC3121432 | biostudies-literature | 2011 Jul

REPOSITORIES: biostudies-literature