Suppressing glucose uptake and acetic acid production increases membrane protein overexpression in Escherichia coli.

ABSTRACT:

Background

The production of integral membrane spanning proteins (IMP's) constitutes a bottleneck in pharmaceutical development. It was long considered that the state-of-the-art was to produce the proteins as inclusion bodies using a powerful induction system. However, the quality of the protein was compromised and the production of a soluble protein that is incorporated into the membrane from which it is extracted is now considered to be a better method. Earlier research has indicated that a slower rate of protein synthesis might overcome the tendency to form inclusion bodies. We here suggest the use of a set of E. coli mutants characterized by a slower rate of growth and protein synthesis as a tool for increasing the amount of soluble protein in high- throughput protein productio

SUBMITTER: Backlund E 

PROVIDER: S-EPMC3121589 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature