Genotype calling in tetraploid species from bi-allelic marker data using mixture models.

ABSTRACT:

Background

Automated genotype calling in tetraploid species was until recently not possible, which hampered genetic analysis. Modern genotyping assays often produce two signals, one for each allele of a bi-allelic marker. While ample software is available to obtain genotypes (homozygous for either allele, or heterozygous) for diploid species from these signals, such software is not available for tetraploid species which may be scored as five alternative genotypes (aaaa, baaa, bbaa, bbba and bbbb; nulliplex to quadruplex).

Results

We present a novel algorithm, implemented in the R package fitTetra, to assign genotypes for bi-allelic markers to tetraploid samples from genotyping assays that produce intensity signals for both alleles. The algorithm is based on the fitting of se

SUBMITTER: Voorrips RE 

PROVIDER: S-EPMC3121645 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature