MiRNA studies in in vitro and in vivo activated hepatic stellate cells.

ABSTRACT:

Aim

To understand which and how different miRNAs are implicated in the process of hepatic stellate cell (HSC) activation.

Methods

We used microarrays to examine the differential expression of miRNAs during in vitro activation of primary HSCs (pHSCs). The transcriptome changes upon stable transfection of rno-miR-146a into an HSC cell line were studied using cDNA microarrays. Selected differentially regulated miRNAs were investigated by quantitative real-time polymerase chain reaction during in vivo HSC activation. The effect of miRNA mimics and inhibitor on the in vitro activation of pHSCs was also evaluated.

Results

We found that 16 miRNAs were upregulated and 26 were downregulated significantly in 10-d in vitro activated pHSCs in comparison to quiescent pHSCs. Overex

SUBMITTER: Maubach G 

PROVIDER: S-EPMC3122263 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature