Rapid molecular strategy for orbivirus detection and characterization.

ABSTRACT: Orbiviruses infect a wide range of hosts, including humans. The ability to detect them has been hampered by their diversity. Here we present a simple consensus reverse transcription (RT)-PCR method targeting the polymerase gene for orbivirus recognition and characterization. Phylogenetic assignment is achieved by automated Web-based sequence analysis of amplification products.

SUBMITTER: Palacios G 

PROVIDER: S-EPMC3122741 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature