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Simultaneous detection of influenza A, influenza B, and respiratory syncytial viruses and subtyping of influenza A H3N2 virus and H1N1 (2009) virus by multiplex real-time PCR.

ABSTRACT: A multiplex real-time PCR assay was developed to simultaneously detect and discriminate influenza A virus subtypes, including novel H1N1 (2009) and seasonal H3N2 virus, influenza B virus, and respiratory syncytial virus (RSV) in a single test tube, with detection sensitivity and specificity of 99% and 100%, respectively, for the four pathogens.

SUBMITTER: Chen Y 

PROVIDER: S-EPMC3122825 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature

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Simultaneous detection of influenza A, influenza B, and respiratory syncytial viruses and subtyping of influenza A H3N2 virus and H1N1 (2009) virus by multiplex real-time PCR.

Chen Yu Y   Cui Dawei D   Zheng Shufa S   Yang Shigui S   Tong Jia J   Yang Dagan D   Fan Jian J   Zhang Jie J   Lou Bin B   Li Xuefen X   Zhuge Xiaoling X   Ye Bo B   Chen Baode B   Mao Weilin W   Tan Yajun Y   Xu Genyun G   Chen Zhenjin Z   Chen Nan N   Li Lanjuan L  

Journal of clinical microbiology 20110126 4

A multiplex real-time PCR assay was developed to simultaneously detect and discriminate influenza A virus subtypes, including novel H1N1 (2009) and seasonal H3N2 virus, influenza B virus, and respiratory syncytial virus (RSV) in a single test tube, with detection sensitivity and specificity of 99% and 100%, respectively, for the four pathogens. ...[more]

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