Mutagenesis Objective Search and Selection Tool (MOSST): an algorithm to predict structure-function related mutations in proteins.

ABSTRACT:

Background

Functionally relevant artificial or natural mutations are difficult to assess or predict if no structure-function information is available for a protein. This is especially important to correctly identify functionally significant non-synonymous single nucleotide polymorphisms (nsSNPs) or to design a site-directed mutagenesis strategy for a target protein. A new and powerful methodology is proposed to guide these two decision strategies, based only on conservation rules of physicochemical properties of amino acids extracted from a multiple alignment of a protein family where the target protein belongs, with no need of explicit structure-function relationships.

Results

A statistical analysis is performed over each amino acid position in the multiple protein alignmen

SUBMITTER: Olivera-Nappa A 

PROVIDER: S-EPMC3123232 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature