Neonatal mouse-derived engineered cardiac tissue: a novel model system for studying genetic heart disease.

ABSTRACT:

Rationale

Cardiomyocytes cultured in a mechanically active 3-dimensional configuration can be used for studies that correlate contractile performance to cellular physiology. Current engineered cardiac tissue (ECT) models use cells derived from either rat or chick hearts. Development of a murine ECT would provide access to many existing models of cardiac disease and open the possibility of performing targeted genetic manipulation with the ability to directly assess contractile and molecular variables.

Objective

To generate, characterize, and validate mouse ECT with a physiologically relevant model of hypertrophic cardiomyopathy.

Methods and results

We generated mechanically integrated ECT using isolated neonatal mouse cardiac cells derived from both wild-type and myosi

SUBMITTER: de Lange WJ 

PROVIDER: S-EPMC3123426 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature