Induction of Th1Immune responses following laser ablation in a murine model of colorectal liver metastases.

ABSTRACT:

Background

Preliminary experimental studies have suggested that the in situ destruction of tumor tissue by local laser ablation (LA) may also stimulate host immunity against cancer. We investigated local and systemic induction of immune responses after laser ablation in the setting of residual tumor.

Methods

A murine colorectal cancer (CRC) liver metastasis model was used. Selected tumors of liver CRC bearing mice and livers of mice without tumor induction were treated with LA. Liver and tumor tissues from the ablation sites and from distant sites were collected at various time points following LA and changes in CD3+ T cells and Kupffer cells (F4/80 marker) infiltration and the expression of interferon gamma (IFNγ) were investigated by immunohistochemistry and ELISpot. Base

SUBMITTER: Lin WX 

PROVIDER: S-EPMC3123581 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature