Evaluation of formalin-fixed paraffin-embedded tissues in the proteomic analysis of parathyroid glands.

ABSTRACT:

Background

Proteomic research in the field of parathyroid tissues is limited by the very small dimension of the glands and by the low incidence of cancer lesions (1%). Formalin-fixed paraffin-embedded (FFPE) tissue specimens are a potentially valuable resource for discovering protein cancer biomarkers. In this study we have verified the applicability of a heat induced protein extraction from FFPE parathyroid adenoma tissues followed by a gel-based or gel-free proteomic approach in order to achieve protein separation and identification.

Results

The best results for high quality MS spectra and parameters, were obtained by using a gel-free approach, and up to 163 unique proteins were identified. Similar results were obtained by applying both SDS-out and SDS-out + TCA/Acetone te

SUBMITTER: Donadio E 

PROVIDER: S-EPMC3123619 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature