Fosmid library end sequencing reveals a rarely known genome structure of marine shrimp Penaeus monodon.

ABSTRACT:

Background

The black tiger shrimp (Penaeus monodon) is one of the most important aquaculture species in the world, representing the crustacean lineage which possesses the greatest species diversity among marine invertebrates. Yet, we barely know anything about their genomic structure. To understand the organization and evolution of the P. monodon genome, a fosmid library consisting of 288,000 colonies and was constructed, equivalent to 5.3-fold coverage of the 2.17 Gb genome. Approximately 11.1 Mb of fosmid end sequences (FESs) from 20,926 non-redundant reads representing 0.45% of the P. monodon genome were obtained for repetitive and protein-coding sequence analyses.

Results

We found that microsatellite sequences were highly abundant in the P. monodon genome, comprising 8.3

SUBMITTER: Huang SW 

PROVIDER: S-EPMC3124438 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature