Chromosome-wide mapping of DNA methylation patterns in normal and malignant prostate cells reveals pervasive methylation of gene-associated and conserved intergenic sequences.

ABSTRACT:

Background

DNA methylation has been linked to genome regulation and dysregulation in health and disease respectively, and methods for characterizing genomic DNA methylation patterns are rapidly emerging. We have developed/refined methods for enrichment of methylated genomic fragments using the methyl-binding domain of the human MBD2 protein (MBD2-MBD) followed by analysis with high-density tiling microarrays. This MBD-chip approach was used to characterize DNA methylation patterns across all non-repetitive sequences of human chromosomes 21 and 22 at high-resolution in normal and malignant prostate cells.

Results

Examining this data using computational methods that were designed specifically for DNA methylation tiling array data revealed widespread methylation of both gene pr

SUBMITTER: Yegnasubramanian S 

PROVIDER: S-EPMC3124442 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature