Normalization in MALDI-TOF imaging datasets of proteins: practical considerations.

ABSTRACT: Normalization is critically important for the proper interpretation of matrix-assisted laser desorption/ionization (MALDI) imaging datasets. The effects of the commonly used normalization techniques based on total ion count (TIC) or vector norm normalization are significant, and they are frequently beneficial. In certain cases, however, these normalization algorithms may produce misleading results and possibly lead to wrong conclusions, e.g. regarding to potential biomarker distributions. This is typical for tissues in which signals of prominent abundance are present in confined areas, such as insulin in the pancreas or β-amyloid peptides in the brain. In this work, we investigated whether normalization can be improved if dominant signals are excluded from the calculation. Because manual i

SUBMITTER: Deininger SO 

PROVIDER: S-EPMC3124646 | biostudies-literature | 2011 Jul

REPOSITORIES: biostudies-literature