Comprehensive expressional analyses of antisense transcripts in colon cancer tissues using artificial antisense probes.

ABSTRACT:

Background

Recent studies have identified thousands of sense-antisense gene pairs across different genomes by computational mapping of cDNA sequences. These studies have shown that approximately 25% of all transcriptional units in the human and mouse genomes are involved in cis-sense-antisense pairs. However, the number of known sense-antisense pairs remains limited because currently available cDNA sequences represent only a fraction of the total number of transcripts comprising the transcriptome of each cell type.

Methods

To discover novel antisense transcripts encoded in the antisense strand of important genes, such as cancer-related genes, we conducted expression analyses of antisense transcripts using our custom microarray platform along with 2376 probes designed specifi

SUBMITTER: Saito R 

PROVIDER: S-EPMC3125192 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature