Quantitative metric profiles capture three-dimensional temporospatial architecture to discriminate cellular functional states.

ABSTRACT:

Background

Computational analysis of tissue structure reveals sub-visual differences in tissue functional states by extracting quantitative signature features that establish a diagnostic profile. Incomplete and/or inaccurate profiles contribute to misdiagnosis.

Methods

In order to create more complete tissue structure profiles, we adapted our cell-graph method for extracting quantitative features from histopathology images to now capture temporospatial traits of three-dimensional collagen hydrogel cell cultures. Cell-graphs were proposed to characterize the spatial organization between the cells in tissues by exploiting graph theory wherein the nuclei of the cells constitute the nodes and the approximate adjacency of cells are represented with edges. We chose 11 different ce

SUBMITTER: McKeen-Polizzotti L 

PROVIDER: S-EPMC3125246 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature