Cell-free synthesis of a functional G protein-coupled receptor complexed with nanometer scale bilayer discs.

ABSTRACT:

Background

G protein coupled receptors (GPCRs) represent the largest family of membrane proteins in the human genome and the richest source of targets for the pharmaceutical industry. A major limitation to characterizing GPCRs has been the difficulty in developing high-level heterologous expression systems that are cost effective. Reasons for these difficulties include inefficient transport and insertion in the plasma membrane and cytotoxicity. Additionally, GPCR purification requires detergents, which have a negative effect on receptor yields and stability.

Results

Here we report a detergent-free cell-free protein expression-based method to obtain pharmacologically active GPCRs in about 2 hours. Our strategy relies on the co-translational insertion of modified GPCRs into na

SUBMITTER: Yang JP 

PROVIDER: S-EPMC3125327 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature