Antibodies on demand: a fast method for the production of human scFvs with minimal amounts of antigen.

ABSTRACT:

Background

Antibodies constitute a powerful tool to study protein function, protein localization and protein-protein interactions, as well as for diagnostic and therapeutic purposes. High-throughput antibody development requires faster methodologies with lower antigen consumption.

Results

Here, we describe a novel methodology to select human monoclonal recombinant antibodies by combining in vitro protein expression, phage display antibody libraries and antibody microarrays. The application of this combination of methodologies permitted us to generate human single-chain variable fragments (scFvs) against two proteins: green fluorescent protein (GFP) and thioredoxin (Trx) in a short time, using as low as 5 μg of purified protein. These scFvs showed specific reactivity against

SUBMITTER: Babel I 

PROVIDER: S-EPMC3125328 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature