Visualization of cellular phosphoinositide pools with GFP-fused protein-domains.

ABSTRACT: This unit describes the method of following phosphoinositide dynamics in live cells. Inositol phospholipids have emerged as universal signaling molecules present in virtually every membrane of eukaryotic cells. Phosphoinositides are present in only tiny amounts as compared to structural lipids, but they are metabolically very active as they are produced and degraded by the numerous inositide kinase and phosphatase enzymes. Phosphoinositides control the membrane recruitment and activity of many membrane protein signaling complexes in specific membrane compartments, and they have been implicated in the regulation of a variety of signaling and trafficking pathways. It has been a challenge to develop methods that allow detection of phosphoinositides at the single-cell level. The only available

SUBMITTER: Balla T 

PROVIDER: S-EPMC3125592 | biostudies-literature | 2009 Mar

REPOSITORIES: biostudies-literature