Biochemical properties and biological function of a monofunctional microbial biotin protein ligase.

ABSTRACT: Biotin protein ligases constitute a family of enzymes that catalyze the linkage of biotin to biotin-dependent carboxylases. In bacteria, these enzymes are functionally divided into two classes: the monofunctional enzymes that catalyze only biotin addition and the bifunctional enzymes that also bind to DNA to regulate transcription initiation. Biochemical and biophysical studies of the bifunctional Escherichia coli ligase suggest that several properties of the enzyme have evolved to support its additional regulatory role. Included among these properties are the order of substrate binding and linkage between the oligomeric state and ligand binding. To test this hypothesized relationship between functionality and biochemical properties in ligases, we have conducted studies of the monofunction

SUBMITTER: Daniels KG 

PROVIDER: S-EPMC3126109 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature