Analysis of poliovirus protein 3A interactions with viral and cellular proteins in infected cells.

ABSTRACT: Poliovirus proteins 3A and 3AB are small, membrane-binding proteins that play multiple roles in viral RNA replication complex formation and function. In the infected cell, these proteins associate with other viral and cellular proteins as part of a supramolecular complex whose structure and composition are unknown. We isolated viable viruses with three different epitope tags (FLAG, hemagglutinin [HA], and c-myc) inserted into the N-terminal region of protein 3A. These viruses exhibited growth properties and characteristics very similar to those of the wild-type, untagged virus. Extracts prepared from the infected cells were subjected to immunoaffinity purification of the tagged proteins by adsorption to commercial antibody-linked beads and examined after elution for cellular and other vira

SUBMITTER: Teterina NL 

PROVIDER: S-EPMC3126254 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature